CMM Flow Cytometry Facility
Cell analysis and cell sorting by flow cytometry is a well established technique whereby rapid measurements of multiple cellular parameters such as light scatter and fluorescence are made on particles or cells as they flow through a sensing point forming the basis for selected populations to be analyzed and/ or sorted out.
The CMM flow Cytometry facility is equipped with a MoFlo high-speed cell sorter with 2 lasers: a dual line Argon 488nm and a HeNe 635nm. Sorting speed is between 10,000 – 50,000 cells/second. Up to four different cell populations can be sorted out simultaneously into tubes or onto plates.
In addition there is a CyAn flow cytometry analyzer equipped with 3 lasers: 488nm, 635nm, and 405nm. It has 11 parameters available for analysis, FS, SS, and 9 colors.
Key applications include multicolor immmunophenotyping, cell cycle analysis, intracellular cytokine detection, apoptosis, cell proliferation analysis, and intracellular calcium measurement. Assistance and guidance in planning your experiments as well as suggestions for helping you achieve optimal results are available.
Annika van Vollenhoven
Location: CMM floor 03, room 055
Phone: +46 8-517 75693
Flow Cytometry Facility
171 76 Stockholm