About KIGene
The former core facilities for DNA sequencing and genotyping at CMM L8:00, and the DNA sequence facility, KISeq, established at CMB 1992, merged year 2008 into the new core facility KIGene - genetic analysis at CMM. We are located at the Department of Molecular Medicine and Surgery, the Neurogenetics Unit, CMM L8:00, Karolinska University Hospital at Solna.
With experiences from two decades of running a core facility we have accumulated much knowledge and proficiency in how to perform services, superwise and support the users. Our vision is to provide a high quality, reliable, high efficiency and flexible core facility, aiming at providing the right solution and study set-up for the right project.
In order to meet the increasing demand on higher throughput and efficiency, as well as on cheaper analyses, our core-facility has introduced several options for gene expression analysis and the analysis of genetic variations including SNPs, microsatellite markers and insertion/deletion polymorphisms.
Our equipment can be used by those who have sufficient knowledge and experience. The facility provides start up theoretical and technical assistance. For some applications, we also offer full-service.
We offer:
- • Sanger sequencing services with turn-around time within the same day
- • Genotyping (SNP and fragment analysis).
- • Expression analysis.
- • Quantity and quality determination of RNA, DNA, protein and dye incorporation.
- • DNA methylation analysis
- • Customer support
Flexible services - three service levels;
• "Hands-on" service: The user receives a short introduction by experienced personnel regarding sample preparation and operation of the instruments. Each user reserves a date and time for occupying an instrument by a booking system.
• "Hands-off" service: The user provides us with pre-made sequence or fragment analysis reactions and we are performing the analysis. The resulting files are transferred to each user by a FTP account or e-mail.
• Full-service: The user provides us with starting materials- as blood, saliva, biopsies, DNA, oligonucleotides- and we are performing the analysis. The resulting files are transferred to each user by a FTP account or e-mail.
Instruments and methods at KIGene
In the table below you can see what the different instruments can be used for. More information about each instrument, including price lists, is available in pdf-format if you follow the links under the table.
- DNA sequence analysis
- fragment analysis
- real-time gene expression analysis,
- SNP analysis (end-point analysis)
- real-time gene expression analysis,
- SNP analysis (end-point analysis)
- single, duplex or triplex SNP analysis
- microsatellite scoring
- haplotyping
- CpG-methylation analysis
- allelic frequency quantification
- short sequence analysis
- small volume quantity and quality determination of DNA, RNA and proteins
- small volume quality determination of DNA and RNA
Address:
KIGene
MMK, Neurogenetics Unit, CMM L8:00
Karolinska University Hospital at Solna, 171 76 Stockholm
E-mail:
kigene@mmk.ki.se
Additional Web page
Information about DNA sequencing, KIGene trouble-shooting and order forms: http://www.ki.se/kiseq/
Web pages to other core facilities at KI
Information about genotyping and expression analysis services at other platforms than offered by KIGene:
http://www.maf.ki.se (Sequenom and Illumina)
http://www.bea.ki.se (Affymetrix and Illumina)