MicroRNA and wound healing – Xu Landén

Research profile

 microRNAs (miRNAs) are ~22 nt noncoding RNAs that can bind to the 3 untranslated region (UTR) of the target messenger RNA (mRNA) and result in translational repression or degradation of mRNA. They are proposed to regulate ~60% of all protein-coding genes in humans. Deregulation of miRNA expression has been shown to contribute many diseases. Recent studies using animal models demonstrated that modulation of miRNA expression by administration of specific miRNA mimics / inhibitors had beneficial effect on diseases. Thus, miRNAs represent targets for potential therapeutic agents. My research focus on the role of miRNAs in skin biology and skin diseases.

Project 1. The role of miRNAs in psoriasis

Psoriasis is a lifelong disease with spontaneous remissions and exacerbations, affecting the patients life quality substantially. Severe psoriasis is a systemic disease with significant comorbidities, such as psoriatic arthritis, metabolic syndrome and cardiovascular disease. Psoriasis shares pathogenetic similarities with other immune-mediated diseases such as Crohn’s disease, rheumatoid arthritis and multiple sclerosis. Thus, psoriasis may be used as a model disease to understand disease mechanisms and test new concepts in chronic inflammatory diseases. We previously identified a distinct miRNA expression profile in psoriasis skin compared with healthy skin. Our current study aims at identifying the putative role(s) of these psoriasis related miRNAs in skin, using materials from a large biobank of psoriasis patients in Stockholm.

Project 2. The role of miRNAs in human skin wound healing

Wound healing is one of the most fundamental physiological processes to keep the integrity of the skin; fails of healing result in chronic wounds, which are a common medical problem with high morbidity and mortality. The molecular pathogenesis of chronic wounds remains poorly understood which impedes the development of effective treatment. We have previously shown that miRNAs play important roles in the regulation of multiple biological functions of keratinocyte and immune cells in skin. In this project we aim to extend our earlier findings and reveal the role of miRNA in wound healing. Furthermore, we will investigate how miRNAs are involved in the pathogenesis of chronic hard-to-heal wounds and explore the potential of miRNA-based therapy for skin wounds.

Methods

In situ hybridization, Immunohistochemistry, Western blotting, Real-time PCR, Primary cell culture, Microarray, Flow Cytometry, Luciferase reporter assay.

Research funding

My research is kindly supported by

  • Svenska Läkaresällskapets fonder
  • European Skin Research Foundation
  • Svenska Sällskapet för Medicinsk Forskning
  • Pfizer AB
  • Stiftelsen Psoriasisfonden
  • HudFonden
  • Karolinska Institutet
  • Tore Nilssons Stiftelse
  • Stiftelsen Lars Hiertas Minne
  • Stiftelsen Sigurd och Elsa Goljes Minne

 

Om du skulle vilja stödja detta forskningsområde kan du göra det via följande konton:

BG 628-4418
PG 514114-8
Ange: Xu Landén

Om du befinner dig utanför Sverige, vänligen använd följande information:

Bank: SEB, Stockholm, Sweden
Kontonummer: 5201-11 370 12
Iban-nummer: SE16 5000 0000 0520 1113 7012
Bic-kod (bankens elektroniska adress): ESSESESS
Kontoinnehavare: Stiftelsen Centrum för Molekylär Medicin
L8:05, Karolinska Universitetssjukhuset
171 76 Stockholm, Sweden

CMM Research Groups

NEUROGENETIK OCH PSYKIATRISKA SJUKDOMAR

GENETISKA SJUKDOMAR

INFLAMMATORISKA SJUKDOMAR

KARDIOVASKULÄRA OCH METABOLA SJUKDOMAR