miRNA and cancer – Pivarcsi/Sonkoly
Our primary interest is the role of microRNAs in the pathogenesis of non-melanoma skin cancers. MiRNAs are short, endogenous RNAs that regulate gene expression at the posttranscriptional level and have critical functions in health and disease. Non-melanoma skin cancers, basal cell carcinomas (BCCs) and squamous cell carcinomas (SCCs) are the most common cancers in human. Until recently, very little was known about the expression and function of miRNAs in human skin and its diseases.
We recently performed the first global analysis of microRNA expression profile in non-melanoma skin cancers and identified a set of microRNAs that may play important roles in their pathogenesis. Our goal is to understand their function both in cancer and under physiological conditions and to determine whether they may become therapeutic targets in the future.
We use TaqMan Low-Density Arrays for microRNA expression profiling and quantitative real-time PCR to measure expression of individual microRNAs in healthy or cancer samples. We use in situ hybridization to learn about the distribution of microRNAs within the tissues. We modulate the activity of miRNAs in cancer cells by using microRNA precursors to overexpress them or inhibitors to suppress them in order to better understand their functions. We study the effect of altered microRNA activity on the phenotype of cancer cell lines using cell cycle progression, apoptosis, colony formation, wound healing, migration and invasion assays. We identify microRNA target genes by using a combination of gene expression profiling, computational approaches, western-blot- and 3UTR luciferase reporter assays.
Om du skulle vilja stödja detta forskningsområde kan du göra det via följande konton:
Ange: Andor Pivarcsi
Om du befinner dig utanför Sverige, vänligen använd följande information:
Bank: SEB, Stockholm, Sweden
Kontonummer: 5201-11 370 12
Iban-nummer: SE16 5000 0000 0520 1113 7012
Bic-kod (bankens elektroniska adress): ESSESESS
Kontoinnehavare: Stiftelsen Centrum för Molekylär Medicin
L8:05, Karolinska Universitetssjukhuset
171 76 Stockholm, Sweden