KIGene – genetic analysis at CMM

KIGene – genetic analysis at CMM

About KIGene

The former core facilities for DNA sequencing and genotyping at CMM L8:00, and the DNA sequence facility, KISeq, established at CMB 1992, merged year 2008 into the new core facility KIGene – genetic analysis at CMM. We are located at the Department of Molecular Medicine and Surgery, the Neurogenetics Unit, CMM L8:00, Karolinska University Hospital at Solna.

With experiences from two decades of running a core facility we have accumulated much knowledge and proficiency in how to perform services, superwise and support the users. Our vision is to provide a high quality, reliable, high efficiency and flexible core facility, aiming at providing the right solution and study set-up for the right project.

In order to meet the increasing demand on higher throughput and efficiency, as well as on cheaper analyses, our core-facility has introduced several options for DNA sequencing, gene expression analysis and the analysis of genetic variations including SNPs, microsatellite markers, copy number variation and insertion/deletion polymorphisms.

Our equipment can be used by those who have sufficient knowledge and experience. The facility provides start up technical assistance. For some applications, we also offer full-service.

We offer

• Sanger sequencing services with turn-around time within the same day
• Genotyping (SNP and fragment analysis)
• Gene expression analysis
• Copy number variation
• Digital-PCR analysis
• miRNA profiling
• Methylation analysis
• Quantity and quality determination of RNA, DNA, protein and dye incorporation
• Customer support

Flexible services – three service levels

• ”Hands-on” service: The user receives a short introduction by experienced personnel regarding sample preparation and operation of the instruments. Each user reserves a date and time for occupying an instrument by a booking system.
• ”Hands-off” service: The user provides us with pre-made sequence or fragment analysis reactions and we are performing the analysis. The resulting files are transferred to each user by e-mail.
• Full-service: The user provides us with starting materials- as blood, saliva, biopsies, DNA, oligonucleotides- and we are performing the analysis. The resulting files are transferred to each user by e-mail.

Instruments and methods at KIGene

In the table below you can see what the different instruments can be used for. More information about each instrument, including price lists, is available in pdf-format if you follow the links under the table.

ABI 3730 PRISM® DNA Analyzers

– Sanger DNA sequence analysis
– Fragment analysis

• Ion Proton Sequencer

– Next Generation Sequencing

Please contact us for more information

• NanoString nCounter analysis system (pdf)

– Gene expression analysis
– copy number variation
– miRNA profiling

QuantStudioTM 6 and 7 FLEX instrument (pdf)

– real-time gene expression analysis,
– SNP analysis (real-time and/or end-point analysis)

• QuantStudioTM 3D (pdf)

– absolute quantification of nucleic acids by digital-PCR
– copy number variation
– gene expression analysis
– rare sequence detection

• Pyromark Q96ID instrument (pdf)

– pyrosequencing, short sequence analysis
– single, duplex or triplex SNP analysis
– microsatellite scoring
– haplotyping
– CpG-methylation analysis
– allelic frequency quantification

NanoDropTM 1000 spectrophotometer (pdf)

– small volume quantity and quality determination of DNA, RNA and proteins

Agilent 2100 Bioanalyzer (pdf)

– small volume quality determination of DNA and RNA

 

Address:

KIGene
MMK, Neurogenetics Unit, CMM L8:00
Karolinska University Hospital at Solna, 171 76 Stockholm

E-mail: kigene@mmk.ki.se

Order forms and KIGene trouble shooting guide

Information about DNA sequencing and trouble shooting guide: KIGene troubleshooting

Requirements for full-service Sanger DNA sequencing and primers stocked at KIGene: Requirements for full-service DNA sequence samples and primer list

Order form for full-service Sanger DNA sequencing: KIGene order form-full service sequence samples

Order form for pre-made Sanger sequence reaction: KIGene order form-premade sequence reactions

Order form for pre-made fragment analysis samples: KIGene order form-fragment analysis

Web pages to other core facilities at KI

Information about genotyping and expression analysis services at other platforms than offered by KIGene:

http://www.maf.ki.se (Sequenom and Illumina)

http://www.bea.ki.se (Affymetrix and Illumina)