Single-cell library preparation core

Single-cell library preparation core

Single-cell facility at CMM is strived to provide both full service and advanced equipment access on the self-service base for single cell library preparation.

We currently run Smart-seq2 plate-based full-length protocol, and 3’-tagged droplet sequencing using 10X Chromium Single Cell Controller.

We also welcome users to set up their own automation protocols using our equipments.

 

Other available equipments are:

Tecan Fluent Robotic System (an automated solution for genomic workflows)

Mantis Nano Dispenser

Agilent bioanalyzer

Pippin Gel Extraction Prep System (DNA Size Selection for PCR fragments and NGS, 100bp – 1.5kb).

 

Descriptions

Tecan Fluent Robotic System (an automated solution for genomic workflows)

Tecan fluent 780 at single cell core facility is an advanced automated workstation which can help you with fast dispensing, transferring or removing low volumes of liquids (0.5 μl to 125 μl in the 384-well format, and 0.5 μl to 500 μl in the 96-well format) with high accuracy. It equips with either 8-channel FCA arm or 384/96-multi channel arm which allows various workflows. A separate robotic gripper arm is used for moving plates on the worktable and acquire plates on the deck. Currently, Smart-seq3 protocol is in-built to perform bead purification and liquid transfer. You are welcome to design and optimize your own assays on it! For more technical and practical detail, please visit https://lifesciences.tecan.com/fluent-laboratory-automation-workstation and contact Peri Noori (peri.noori@ki.se).

 

Pippin Gel Extraction Prep System (DNA Size Selection for PCR fragments and NGS, 100bp – 1.5kb).

 

The facility is located at CMM L8:01 room 052.

 

Contact 

Peri Noori, for more information and introduction.

E-mail: peri.noori@ki.se  

 

Scientific advisors

Qiaolin Deng

E-mail: qiaolin.deng@ki.se

Eduardo Villablanca

E-mail: eduardo.villablanca@ki.se