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Flow cytometry

DESCRIPTION:

Cell analysis and cell sorting by flow cytometry is a well established technique whereby rapid measurements of multiple cellular parameters such as light scatter and fluorescence are made on particles or cells as they flow through a sensing point forming the basis for selected populations to be analyzed and/ or sorted out. The CMM flow cytometry facility is equipped with:

Cell sorters:

MoFlo Legacy with 2 lasers (488 and 635 nm)

BD Influx with 5 lasers (488, 561, 641 405, and 355 nm)

Up to six different cell populations can be sorted out simultaneously into tubes, or one population onto plates (including index sorting).

Sony SH800 with 4 lasers (488, 561, 638, and 405 nm)

Up to two different populations can be sorted out simultaneously into tubes, or one population onto plates (including index sorting).

Analyzers:

BD FACSVerse;  equipped with 3 lasers: 488nm, 635nm, and 405nm.

BD LSR Fortessa;  equipped with 5 lasers: 488, 552, 640, 405, and 350 nm.

Cytek Aurora; equipped with 4 lasers: 488nm, 635nm, 560nm, and 405nm.

Cytek Aurora; equipped with 3 lasers: 488nm, 635nm and 405nm.

Key applications include multicolor immmunophenotyping, cell cycle analysis, intracellular cytokine detection, apoptosis, cell proliferation analysis, and intracellular calcium measurement. Assistance and guidance in planning your experiments as well as suggestions for helping you achieve optimal results are available.

CONTACT:

Annika van Vollenhoven

Location: CMM floor 03, room 064

E-mail: annika.van.vollenhoven@ki.se

Phone: +46 8-517 75693

Address:

Flow Cytometry Facility

CMM L8:03

171 76 Stockholm

About CMM

The Center for Molecular Medicine (CMM) is a foundation instituted by the Stockholm County Council (Region Stockholm). CMM is at the heart of a close partnership with the Karolinska University Hospital and Karolinska Institutet, fueling advancements in biomedical and clinical research.

Contact

Center for Molecular Medicine Foundation, org. nr. 815201-3689

Karolinska University Hospital L8:05

Visionsgatan 18

171 76 Stockholm, Sweden

communication@cmm.se

CMM
Karolinska institutet
Karolinska universitetssjukhuset